ydaB [2017-10-10 09:19:20]

similar to acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase)

locus: BSU04170

pI: 4.85

mw: 51.07 kDa

protein length: 465 aa

gene length: 1395 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 469,426 → 470,937

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Structure

• [PDB|3R44] (from Mycobacterium tuberculosis, 33% identity)

Expression and Regulation

Operons

• genes: [gene|2436420C7781DCB61B9A939B47747265E23740A5|ydaB]
  description: [Pubmed|21815947]

  additional information

  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] (the half-life of the mRNA increases from 2 to 80 min) [PubMed|21815947]

Biological materials

Mutant

• BKE04170 (Δ[gene|2436420C7781DCB61B9A939B47747265E23740A5|ydaB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGTCATCTCCTATAT, downstream forward: _UP4_TCATAAAAGAAGAAGCCCGT
• BKK04170 (Δ[gene|2436420C7781DCB61B9A939B47747265E23740A5|ydaB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGTCATCTCCTATAT, downstream forward: _UP4_TCATAAAAGAAGAAGCCCGT

References

• 1460045,21815947